Dopaminergic, glutamatergic but not opioidergic mechanisms mediate induction of FOS-like protein by cocaethylene

Cocaethylene is a psychoactive metabolite formed\ud during the combined consumption of cocaine and ethanol. As\ud this metabolite has many properties in common with cocaine, it is conceivable that cocaethylene administration may induce the activity of nuclear transcription factors that regulate the expression of late-response genes. Therefore, the temporal induction of FOS-like protein in rat brain was examined following IP administration of 60 mmol/kg cocaethylene. Immunoreactivity for the protein was detectable at 1 h in striatal neurons and had virtually disappeared 6 h after drug treatment. Administration of\ud specific dopaminergic (SCH-23390; 0.5 mg/kg) and glutamatergic (MK-801; 1 mg/kg) receptor antagonists prior to cocaethylene indicated a significant role for dopamine (D1) and Nmethyl-D-aspartate receptor subtypes in mediating the nuclear induction of the aforementioned transcription factor protein. In contrast, no significant effects on FOS-like protein in discrete neurons of the caudate putamen were found when spiradoline (U-62066), a kappa opioid-receptor agonist, was administered either IP (10 mg/kg) or directly (50 nmol) into the brain parenchyma. In addition, we uncovered a differential sensitivity of Long–Evans rats to the behavioral effects of cocaethylene, with the psychoactive metabolite producing significantly less behavioral activity (e.g., locomotion, rearing, and continuous sniffing)than that produced by cocaine (molar equivalent of 60 mmol/kg cocaethylene). These findings indicate both common and disparate effects of cocaethylene and its parent compound, cocaine, on receptor pathways that regulate target alterations in gene expression and drug-induced motor behavior

Differential Behavioral Responses to\ud Cocaethylene of Long-Evans and\ud Sprague-Dawley Rats: Role of Serotonin

Cocaethylene is a neuroactive metabolite derived from the concurrent consumption of cocaine and ethanol. The effects of cocaethylene on locomotor activity, stereotypy, and rearing in Long-Evans and Sprague-Dawley rats were compared.A single cocaine injection (molar equivalent of 60 μmol/kg cocaethylene, intraperitoneal) elicited a robust series of motor output behaviors, including locomotion, stereotypy, and rearing over a 30-minute testing period in Long-Evans rats. In contrast, cocaethylene administration,\ud under comparable testing conditions, produced no significant changes in locomotor and investigatory behaviors. Because cocaethylene has relatively little impact on serotonin (5-HT) reuptake as opposed to reuptake of dopamine, we pretreated Long-Evans rats with fluoxetine (10 mg/kg; IP), a selective 5-HT reuptake inhibitor. Fluoxetine profoundly augmented cocaethylene-stimulated behaviors in this rat phenotype. To examine whether other rat strains exhibit a similar response to cocaethylene, Sprague-Dawley rats were injected (IP) with cocaethylene and their behavior patterns monitored over a 30-minute testing period. Cocaethylene produced marked locomotor and exploratory behaviors in this strain, suggesting therefore that Long-Evans and Sprague-\ud Dawley rats differ in their response to cocaethylene. To relate these behavioral differences to possible structural differences in the neuronal density of dopaminergic or\ud serotonergic neurons, Long-Evans and Sprague-Dawley brains were evaluated for tyrosine hydroxylase and 5-HT immunocytochemistry. No gross morphological differences\ud in neuronal architecture or density were found in the ventral tegmental area or dorsal raphe nucleus of the two rat phenotypes. These results indicate that two commonly used rat strains show a differential response to cocaethylene and the neurochemical basis for this behavioral difference may be related to synaptic 5-HT bioavailability

Effects of cocaethylene on dopamine and serotonin synthesis in Long–Evans and Sprague–Dawley brains

We examined the behavioral and neurochemical effects of cocaethylene treatment in Long–Evans (�LE). and Sprague–Dawley� (SD) rats. Cocaethylene-induced behaviors were significantly less in LE rats. Cocaethylene caused an inhibition of dopamine synthesis in the caudate nucleus and nucleus accumbens that was equivalent in both rat lines. Serotonin synthesis was also suppressed by cocaethylene treatment, however this phenomenon was less pronounced when compared with the effects on dopamine synthesis

La educación en valores como estrategia de desarrollo y consolidación de la persona moral

Se plantea la centralidad de los valores a través de una serie de reflexiones sociales y pedagógicas que pretenden conformar las líneas de actuación asociadas al afianzamiento y mejora de la personalidad moral. Resulta urgente procurar un desarrollo moral de la persona que se oriente en dos direcciones complementarias: una, hacia el logro de una felicidad interior no egoísta y otra, hacia la consecución de una felicidad fraternal y hospitalaria

On-the-go yield and sugar sensing in grape harvester

This paper summarises the results of a joint R+D project between university and industry. The study was developed at the Alt Penedès region, in Barcelona, during the 2006 and 2007 (on 3, 22, 69 fields respectively). The quality sensors set-up in year 2007, mounted on a New Holland SB55 grape harvester, were: two load cells, one refractometer, an ambient temperature prove and a GPS antenna, while in 2006 only the load cells and the GPS performed properly. The method used for this study is as follows: 1. Data recording from GPS and Logger (the latter is use for according and digitalising the sensor signal); 2. Wireless download of data to a PC; 3. Automatic data integration in a single file; 4. Lane automatic identification based on trajectory angles, machine forward speed determination, effective time calculation, masic flow, kg/m, and total amount harvested, kg/hopper, computation of characteristic soluble solid content and temperature during harvest; 5. Data broadcasting through GPRS to the winery; 6. Comparison of transmitted data with the invoice of the winery containers. After the season was finished, a data post processing was performed in order to a assess the causes of isolated incidences that were registered in 10 fields. Also a recalibration of the sensors for future seasons was performed. At current stage R 2 of 0.9547 is found between winery and in field yield data. Beside georeference data were gathered and compare to the remote photos in “Instituto Cartográfico de Cataluña”. Site-specific yield maps and speed maps have been computed while broad soluble solid information is not available due to slight dysfunctions of the grape juice pumping system towards to the refractometer

Nilpotent classical mechanics: s-geometry

We introduce specific type of hyperbolic spaces. It is not a general linear covariant object, but of use in constructing nilpotent systems. In the present work necessary definitions and relevant properties of configuration and phase spaces are indicated. As a working example we use a D=2 isotropic harmonic oscillator.Comment: 8 pages, presented at QGIS, June 2006, Pragu

Analytical Expressions for Radiative Opacities of Low Z Plasmas

In this work we obtain analytical expressions for the radiative opacity of several low Z plasmas (He, Li, Be, and B) in a wide range of temperatures and densities. These formulas are obtained by fitting the proposed expression to mean opacities data calculated by using the code ABAKO/ RAPCAL. This code computes the radiative properties of plasmas, both in LTE and NLTE conditions, under the detailed-level-accounting approach. It has been successfully validated in the range of interest in previous works

Greedy seeding procedure for GAs solving a strip packing problem

In this paper, the two-dimensional strip packing problem with 3-stage level patterns is tackled using genetic algorithms (GAs). We evaluate the usefulness of a greedy seeding procedure for creating the initial population, incorporating problem knowledge. This is motivated by the expectation that the seeding will speed up the GA by starting the search in promising regions of the search space. An analysis of the impact of the seeded initial population is offered, together with a complete study of the influence of these modifications on the genetic search. The results show that the use of an appropriate seeding of the initial population outperforms existing GA approaches on all the used problem instances, for all the metrics used, and in fact it represents the new state of the art for this problem.Red de Universidades con Carreras en Informática (RedUNCI